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plvx puro empty vector  (TaKaRa)


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    Structured Review

    TaKaRa plvx puro empty vector
    Plvx Puro Empty Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 336 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plvx puro empty vector/product/TaKaRa
    Average 96 stars, based on 336 article reviews
    plvx puro empty vector - by Bioz Stars, 2026-03
    96/100 stars

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    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Differences in syncytia formation by SARS-CoV-2 variants modify host chromatin accessibility and cellular senescence via TP53

    doi: 10.1016/j.celrep.2023.113478

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Gene inserts from pDONR221-EGFP (Addgene #25899), pDONR207 SARS-CoV-2 nsp3 (Addgene #141257), pDONR223 SARS-CoV-2 nsp6 (Addgene #141260), and pDONR223 SARS-CoV-2 spike (Addgene #149329) were subcloned into pLVpuro-CMV-N-3xFLAG (Addgene #123223) by Gateway cloning. pLVX-EF1a-IRES-puro empty vector was generated by swapping the eGFP insert of pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro (Addgene #141395) with a multiple cloning site. pLVX-EF1a-SARS-CoV-2-nsp16-IRES-puro was generated by subcloning the nsp16 coding sequence from pDONR223 SARS-CoV-2 nsp16 (Addgene #141269) into pLVX-EF1a-IRES-puro empty vector.

    Techniques: FLAG-tag, Luciferase, Virus, Recombinant, SYBR Green Assay, Activity Assay, Software, CRISPR