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plvx puro empty vector  (TaKaRa)


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    Structured Review

    TaKaRa plvx puro empty vector
    Plvx Puro Empty Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 416 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plvx+puro+empty+vector/Lenti-X+Expression+System/pm40638555-54-5-9
    Average 96 stars, based on 416 article reviews
    plvx puro empty vector - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Construct:

    Article Title: ARHGAP10, Transcriptionally Regulated by Sodium Butyrate, Promotes Ferroptosis of Ovarian Cancer Cells.
    Article Snippet: The shRNA was included in pLKO.1 lentiviral vector (Addgene, Watertown, MA, USA). .. Lentiviral constructs of pLKO.1-scramble shRNA (shNC), pLKO.1shARHGAP10, pLVX-Puro empty vector (Clontech, Palo Alto, CA, USA), or pLVX-Puro-ARHGAP10 were co-transfected with viral packaging plasmids (psPAX2 and pMD2.G) into 293T cells. ..

    Article Title: SMAD4 inhibits glycolysis in ovarian cancer through PI3K/AKT/HK2 signaling pathway by activating ARHGAP10.
    Article Snippet: .. Following the manufacturer's instructions, lentiviral constructs of pLKO.1scramble shRNA (shNC), pLKO.1-shARHGAP10, pLVX-Puro empty vector (Clontech, USA), or pLVX-Puro-ARHGAP10 were cotransfected with viral packaging plasmids (psPAX2 and pMD2.G) into 293 T cells using lipofectamine 2000 (Invitrogen). ..

    Article Title: SMAD4 inhibits glycolysis in ovarian cancer through PI3K / AKT / HK2 signaling pathway by activating ARHGAP10
    Article Snippet: .. Following the manufacturer's instructions, lentiviral constructs of pLKO.1‐scramble shRNA (shNC), pLKO.1‐shARHGAP10, pLVX‐Puro empty vector (Clontech, USA), or pLVX‐Puro‐ARHGAP10 were cotransfected with viral packaging plasmids (psPAX2 and pMD2.G) into 293 T cells using lipofectamine 2000 (Invitrogen). ..

    Article Title: Tauroursodeoxycholic acid protects Schwann cells from high glucose-induced cytotoxicity by targeting NLRP3 to regulate cell migration and pyroptosis.
    Article Snippet: .. Lentiviral constructs of pLVX-Puro empty vector (Clontech) as negative control or pLVX-Puro-NLRP3 were cotransfected with viral packaging plasmids (psPAX2 and pMD2.G) into 293T cells using Lipofectamine 2000 (Invitrogen), following the supplier’s procedure. .. Lentiviral constructs of pLVX-Puro empty vector (Clontech) as negative control or pLVX-Puro-NLRP3 were cotransfected with viral packaging plasmids (psPAX2 and pMD2.G) into 293T cells using Lipofectamine 2000 (Invitrogen), following the supplier’s procedure.

    shRNA:

    Article Title: ARHGAP10, Transcriptionally Regulated by Sodium Butyrate, Promotes Ferroptosis of Ovarian Cancer Cells.
    Article Snippet: The shRNA was included in pLKO.1 lentiviral vector (Addgene, Watertown, MA, USA). .. Lentiviral constructs of pLKO.1-scramble shRNA (shNC), pLKO.1shARHGAP10, pLVX-Puro empty vector (Clontech, Palo Alto, CA, USA), or pLVX-Puro-ARHGAP10 were co-transfected with viral packaging plasmids (psPAX2 and pMD2.G) into 293T cells. ..

    Article Title: SMAD4 inhibits glycolysis in ovarian cancer through PI3K/AKT/HK2 signaling pathway by activating ARHGAP10.
    Article Snippet: .. Following the manufacturer's instructions, lentiviral constructs of pLKO.1scramble shRNA (shNC), pLKO.1-shARHGAP10, pLVX-Puro empty vector (Clontech, USA), or pLVX-Puro-ARHGAP10 were cotransfected with viral packaging plasmids (psPAX2 and pMD2.G) into 293 T cells using lipofectamine 2000 (Invitrogen). ..

    Article Title: SMAD4 inhibits glycolysis in ovarian cancer through PI3K / AKT / HK2 signaling pathway by activating ARHGAP10
    Article Snippet: .. Following the manufacturer's instructions, lentiviral constructs of pLKO.1‐scramble shRNA (shNC), pLKO.1‐shARHGAP10, pLVX‐Puro empty vector (Clontech, USA), or pLVX‐Puro‐ARHGAP10 were cotransfected with viral packaging plasmids (psPAX2 and pMD2.G) into 293 T cells using lipofectamine 2000 (Invitrogen). ..

    Stable Transfection:

    Article Title: Exploring the ATG9A interactome uncovers interaction with VPS13A
    Article Snippet: .. To generate stable cell lines using the ATG2A/B DKO cell line, cell cultures were transduced with Lenti-virus containing empty PLVX-Puro (empty) vector (Takara Bio, cat. no. 632164), PLVX-Puro-ATG2A WT or PLVX-ATG2A-VPS13A chimera. ..

    Article Title: ATG9A and ATG2A form a heteromeric complex essential for autophagosome formation.
    Article Snippet: .. To generate stable cell lines using the ATG2A/B DKO cell line, cell cultures were transduced with Lenti-virus containing empty PLVX-Puro (empty) vector (Takara Bio, Cat. No. 632164), PLVX-Puro-ATG2A WT or PLVX-ATG2A DS2. ..

    Transduction:

    Article Title: Exploring the ATG9A interactome uncovers interaction with VPS13A
    Article Snippet: .. To generate stable cell lines using the ATG2A/B DKO cell line, cell cultures were transduced with Lenti-virus containing empty PLVX-Puro (empty) vector (Takara Bio, cat. no. 632164), PLVX-Puro-ATG2A WT or PLVX-ATG2A-VPS13A chimera. ..

    Article Title: XML Attenuates Ox-LDL-Induced Endothelial Progenitor Cell Senescence via Gria2 and cAMP Pathways.
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 3 of 14 (Sigma–Aldrich, Cat# P8833) for 7 days. .. Control cells were transduced with pLVX- Puro empty vector (Takara Bio, Cat. No. 632164) or pLVX- Puro- GFP lentivirus. .. Male Sprague–Dawley rats (8–10 weeks old, 250–300 g) were purchased from Charles River Laboratories, Wilmington, MA, USA, and housed at 22°C ± 2°C with a 12- h light/dark cycle, 50%–60% humidity, and ad libitum access to food and water.

    Article Title: XML Attenuates Ox‐ LDL ‐Induced Endothelial Progenitor Cell Senescence via Gria2 and cAMP Pathways
    Article Snippet: EPCs were transduced and selected with 2 μg/mL puromycin (Sigma–Aldrich, Cat# P8833) for 7 days. .. Control cells were transduced with pLVX‐Puro empty vector (Takara Bio, Cat. No. 632164) or pLVX‐Puro‐GFP lentivirus. .. Male Sprague–Dawley rats (8–10 weeks old, 250–300 g) were purchased from Charles River Laboratories, Wilmington, MA, USA, and housed at 22°C ± 2°C with a 12‐h light/dark cycle, 50%–60% humidity, and ad libitum access to food and water.

    Article Title: ATG9A and ATG2A form a heteromeric complex essential for autophagosome formation.
    Article Snippet: .. To generate stable cell lines using the ATG2A/B DKO cell line, cell cultures were transduced with Lenti-virus containing empty PLVX-Puro (empty) vector (Takara Bio, Cat. No. 632164), PLVX-Puro-ATG2A WT or PLVX-ATG2A DS2. ..

    Control:

    Article Title: XML Attenuates Ox-LDL-Induced Endothelial Progenitor Cell Senescence via Gria2 and cAMP Pathways.
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 3 of 14 (Sigma–Aldrich, Cat# P8833) for 7 days. .. Control cells were transduced with pLVX- Puro empty vector (Takara Bio, Cat. No. 632164) or pLVX- Puro- GFP lentivirus. .. Male Sprague–Dawley rats (8–10 weeks old, 250–300 g) were purchased from Charles River Laboratories, Wilmington, MA, USA, and housed at 22°C ± 2°C with a 12- h light/dark cycle, 50%–60% humidity, and ad libitum access to food and water.

    Article Title: XML Attenuates Ox‐ LDL ‐Induced Endothelial Progenitor Cell Senescence via Gria2 and cAMP Pathways
    Article Snippet: EPCs were transduced and selected with 2 μg/mL puromycin (Sigma–Aldrich, Cat# P8833) for 7 days. .. Control cells were transduced with pLVX‐Puro empty vector (Takara Bio, Cat. No. 632164) or pLVX‐Puro‐GFP lentivirus. .. Male Sprague–Dawley rats (8–10 weeks old, 250–300 g) were purchased from Charles River Laboratories, Wilmington, MA, USA, and housed at 22°C ± 2°C with a 12‐h light/dark cycle, 50%–60% humidity, and ad libitum access to food and water.

    Plasmid Preparation:

    Article Title: XML Attenuates Ox-LDL-Induced Endothelial Progenitor Cell Senescence via Gria2 and cAMP Pathways.
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 3 of 14 (Sigma–Aldrich, Cat# P8833) for 7 days. .. Control cells were transduced with pLVX- Puro empty vector (Takara Bio, Cat. No. 632164) or pLVX- Puro- GFP lentivirus. .. Male Sprague–Dawley rats (8–10 weeks old, 250–300 g) were purchased from Charles River Laboratories, Wilmington, MA, USA, and housed at 22°C ± 2°C with a 12- h light/dark cycle, 50%–60% humidity, and ad libitum access to food and water.

    Article Title: XML Attenuates Ox‐ LDL ‐Induced Endothelial Progenitor Cell Senescence via Gria2 and cAMP Pathways
    Article Snippet: EPCs were transduced and selected with 2 μg/mL puromycin (Sigma–Aldrich, Cat# P8833) for 7 days. .. Control cells were transduced with pLVX‐Puro empty vector (Takara Bio, Cat. No. 632164) or pLVX‐Puro‐GFP lentivirus. .. Male Sprague–Dawley rats (8–10 weeks old, 250–300 g) were purchased from Charles River Laboratories, Wilmington, MA, USA, and housed at 22°C ± 2°C with a 12‐h light/dark cycle, 50%–60% humidity, and ad libitum access to food and water.

    Article Title: Tauroursodeoxycholic acid protects Schwann cells from high glucose-induced cytotoxicity by targeting NLRP3 to regulate cell migration and pyroptosis.
    Article Snippet: .. Lentiviral constructs of pLVX-Puro empty vector (Clontech) as negative control or pLVX-Puro-NLRP3 were cotransfected with viral packaging plasmids (psPAX2 and pMD2.G) into 293T cells using Lipofectamine 2000 (Invitrogen), following the supplier’s procedure. .. Lentiviral constructs of pLVX-Puro empty vector (Clontech) as negative control or pLVX-Puro-NLRP3 were cotransfected with viral packaging plasmids (psPAX2 and pMD2.G) into 293T cells using Lipofectamine 2000 (Invitrogen), following the supplier’s procedure.

    Negative Control:

    Article Title: Tauroursodeoxycholic acid protects Schwann cells from high glucose-induced cytotoxicity by targeting NLRP3 to regulate cell migration and pyroptosis.
    Article Snippet: .. Lentiviral constructs of pLVX-Puro empty vector (Clontech) as negative control or pLVX-Puro-NLRP3 were cotransfected with viral packaging plasmids (psPAX2 and pMD2.G) into 293T cells using Lipofectamine 2000 (Invitrogen), following the supplier’s procedure. .. Lentiviral constructs of pLVX-Puro empty vector (Clontech) as negative control or pLVX-Puro-NLRP3 were cotransfected with viral packaging plasmids (psPAX2 and pMD2.G) into 293T cells using Lipofectamine 2000 (Invitrogen), following the supplier’s procedure.



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    Image Search Results


    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Differences in syncytia formation by SARS-CoV-2 variants modify host chromatin accessibility and cellular senescence via TP53

    doi: 10.1016/j.celrep.2023.113478

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Gene inserts from pDONR221-EGFP (Addgene #25899), pDONR207 SARS-CoV-2 nsp3 (Addgene #141257), pDONR223 SARS-CoV-2 nsp6 (Addgene #141260), and pDONR223 SARS-CoV-2 spike (Addgene #149329) were subcloned into pLVpuro-CMV-N-3xFLAG (Addgene #123223) by Gateway cloning. pLVX-EF1a-IRES-puro empty vector was generated by swapping the eGFP insert of pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro (Addgene #141395) with a multiple cloning site. pLVX-EF1a-SARS-CoV-2-nsp16-IRES-puro was generated by subcloning the nsp16 coding sequence from pDONR223 SARS-CoV-2 nsp16 (Addgene #141269) into pLVX-EF1a-IRES-puro empty vector.

    Techniques: FLAG-tag, Luciferase, Virus, Recombinant, SYBR Green Assay, Activity Assay, Software, CRISPR