Journal: Cell reports
Article Title: Differences in syncytia formation by SARS-CoV-2 variants modify host chromatin accessibility and cellular senescence via TP53
doi: 10.1016/j.celrep.2023.113478
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Gene inserts from pDONR221-EGFP (Addgene #25899), pDONR207 SARS-CoV-2 nsp3 (Addgene #141257), pDONR223 SARS-CoV-2 nsp6 (Addgene #141260), and pDONR223 SARS-CoV-2 spike (Addgene #149329) were subcloned into pLVpuro-CMV-N-3xFLAG (Addgene #123223) by Gateway cloning. pLVX-EF1a-IRES-puro empty vector was generated by swapping the eGFP insert of pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro (Addgene #141395) with a multiple cloning site. pLVX-EF1a-SARS-CoV-2-nsp16-IRES-puro was generated by subcloning the nsp16 coding sequence from pDONR223 SARS-CoV-2 nsp16 (Addgene #141269) into pLVX-EF1a-IRES-puro empty vector.
Techniques: FLAG-tag, Luciferase, Virus, Recombinant, SYBR Green Assay, Activity Assay, Software, CRISPR